![]() ![]() Once you press OK, the compensation settings for any plot on your layout to which this compensation was applied will be changed to the new values. If the Compensations window is open and displaying the compensation currently being edited, the Compensation Matrix and Spillover Matrix values will update in real time, as will plots to which the compensation has been applied.Ĭlick Cancel to reject the compensation changes and exit the dialog, or Restore to discard the changes but continue editing the plot. If you check Automatic Preview, the plot will update with the new compensation settings as you edit the compensation in real-time. Move the slider to either increase or decrease the amount of compensation. Use this dialog to compensate the two parameters against each other on the plot. The compensation matrix was calculated in Flowjo. Figure 3: Beads were stained with antibodies and acquired on a FACSCanto-II. The instrument was under tight control, and the voltages based on application-specific settings, coupled with using peak 6 beads to check voltage. Here is a collection of external links about Compensation.Īll of the pages linked above, also link back here for your convenience.Figure 9.11 Creating a New Compensation from a 2D PlotĪfter naming a new compensation and clicking OK, or selecting a compensation definition to edit, the Edit Compensation dialog will appear (Figure 9.13). Figure 3 shows how a compensation matrix acquired one week apart change. When you are ready to proceed to a more complete overview of the new compensation work flow, please see this document.Īlso, consider the Compensation Tutorial. ![]() One other document you will likely find useful is:Īpplying/Renaming – the matrix, a small overview. If, instead, the message reads Calculation Error, This means FlowJo was unable to calculate the matrix from your given controls.
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